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KMID : 0900919970210040397
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Korean journal of Animal Reproduction
1997 Volume.21 No. 4 p.397 ~ p.403
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Titer Amplification of GALV (Gibbon Ape Leukemia Virus) Pseudotyped Retrovirus Vectors Produced from PG13 Cells
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Kim Teo-An
Park Yoon-Yub
Kwon Mo-Seon
Park Young-Seek
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Abstract
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For the ultimate goal of efficient retrovirus vector-mediated transgenic animal production, we tried to increase virus titer by employing three methods: boosting virus production by treating virus-producing cells with sodium butyrate, concentration of virus stock by either filtration or ultracentrifugation. Compared to the control, applications of sodium butyrate (5 mM) treatment and filtration resulted in only 3 and 3. 6 folds of titer increases on bovine EBTr target cells, respectively. However, concentration of virus-containing medium by ultracentrifugation showed 12.5 folds of titer increase compared to the control (10{times}10^5 LacZ^+ TU Im), indicating the best method which can enhance retrovirus vector-mediated transgenic animal production.
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KEYWORD
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Retrovirus vector, Packaging cell, Sodium butyrate, LacZ^+TU/ml, Concentration of virus stock
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